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Knex 78780 - Education DNA Replication Teachers Guide Manual

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Questions/Activities for students:
• Cellularrepairmechanismsexistforeachofthetypesofmutationeventsdescribedabove.
Discuss the importance of the timing of the repair event with respect to replication and transcription.
• Howmightasingle,pointmutationresultinthedeletionofanentireportionofaprotein?
(Change of an amino-acid codon to a stop codon.)
How might a single point mutation result in a protein of twice the length?
(Change of a stop codon into an amino-acid codon with continued read-through to an unrelated
DNA sequence/gene.)
• Somemutationaleventsmayleadtobeneficialoutcomesfortheorganismoranimal.Forexample,
bacteria often become resistant to antibiotics through mutations that change the target site for
the antibiotic without otherwise affecting the essential functions of the bacteria. Such a mutation,
however, is likely to happen to only one out of a billion bacteria. Discuss how quickly one resistant
bacteria might replicate to outnumber a billion others of the original strain if the antibiotic is present.
Assume the bacteria can double every 20 minutes. How long would it take for one mutated
bacterium to replicate to become 1,000,000?
(About 30 doublings, which is only 10 hours!)
useful relAted web sites
http://www.nih.gov/ - NIH Homepage
MODULE III: CODING, TRANSLATION, AND MUTATIONS
LESSON 7
www.knexeducation.com
Education
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